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Peptide Handling Guideline

Guideline for Reconstitution
A effective bioassay project begins with proper peptide handling and solubilization, and we agree that following this handling guideline can help you dissolve your peptides correctly. You will see the reconstitution conditions we used in the peptide purification process on CoA along with each peptide delivery – this is for your reference only; you can dissolve the peptide in a different solvent depending on your assay needs. original site
To test the dissolution process, use only a small aliquot of peptide. Apply to the larger aliquot as desired once you’re satisfied.
In general, the solvent used should be one that will make your experiment simpler or more compatible. However, we must bear in mind that finding a “ideal” solvent that will solubilize peptides, preserve their integrity, and be consistent with biological assays can be difficult at times.
The most suitable solvent should be used as the initial solvent. For example, it is preferable to dissolve a very hydrophobic peptide in a small amount of organic solvent (such as DMSO or acetonitrile) before applying the aqueous solution. To put it another way, applying organic solvent to a hydrophobic peptide suspension in aqueous solution is unlikely to make it dissolve.
At temperatures as low as -20°C, peptide solution can become unstable. As a result, a peptide solution should be used as soon as possible after it has been formulated.
To dissolve my peptides, what solvent(s) can I use?
If it’s a short peptide (less than 5aa), try sterile distilled water first; it’ll probably dissolve.

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